Highly efficient and genotype-independent barley gene editing based on anther culture

Effect of ovary induction on bread wheat anther culture: ovary genotype and developmental stage, and candidate gene association

Ovary pre-conditioned medium and ovary co-culture increased the efficiency of green doubled haploid plant production in bread wheat anther culture. The positive effect of this medium led to a 6- and 11-fold increase in the numbers of embryos and green plants, respectively, having a greater effect on a medium-low responding cultivar. Ovary genotype and developmental stage significantly affected ...

Anther Culture of Pepper (capsicum Annuum L.) : Comparative Study on Effect of the Genotype

There were investigated and compared the in vitro response in anther culture of 16 Bulgarian pepper (Capsicum annuum L.) genotypes: six lines, six varieties and four hybrids developed in Maritsa Vegetable Crops Research Institute, Plovdiv. The anthers reacted with induction of direct embryogenesis or callusogenesis. Only in two of the studied genotypes (line N145 and variety Zlaten medal) the a...

Highly efficient, rapid and co-CRISPR independent genome editing in C. elegans

Highly Efficient, Rapid and Co-CRISPR-Independent Genome Editing in Caenorhabditis elegans

We describe a rapid and highly efficient method to generate point mutations in Caenorhabditis elegans using direct injection of CRISPR-Cas9 ribonucleoproteins. This versatile method does not require sensitized genetic backgrounds or co-CRISPR selection-based methods, and represents a single strategy that can be used for creating genomic point mutations, regardless of location. As proof of princ...

Agent-Based Model of Genotype Editing

Evolutionary algorithms rarely deal with ontogenetic, non-inherited alteration of genetic information because they are based on a direct genotype-phenotype mapping. In contrast, several processes have been discovered in nature which alter genetic information encoded in DNA before it is translated into amino-acid chains. Ontogenetically altered genetic information is not inherited but extensivel...